protogel= volume of 30% ProtoGel, X = per - cent monomer desired in gel and V t = total volume of gel casting solution. EXAMPLE: To make 100 mL of a 10% percent monomer gel, the amount ofThe ProtoGel Sample Prep Kit concentrates proteins as dilute as 25ng100µl. The unique ProtoGel Sample Prep Kit casts the finest net of any recovery system, concentrating